Fig. 3: sCLs containing LGR5-positive cells continuously formed in the invasive front. | Laboratory Investigation

Fig. 3: sCLs containing LGR5-positive cells continuously formed in the invasive front.

From: Continuous formation of small clusters with LGR5-positive cells contributes to tumor growth in a colorectal cancer xenograft model

Fig. 3

a Xenograft model inoculated with LGR5-positive CSC line (PLR123) at day 19 after inoculation. LGR5 (green) and E-cadherin (red). Multiple photos are combined into one image. Scale bar = 100 μm. b Serial sections for sCL in the invasive front at day 19. LGR5 (green) and HLA-DMA (red). Tumor cells are enclosed by dotted line. Number shows the order of serial sections. Scale bar = 50 μm. c Images for xenograft model inoculated with PLR123 line at day 35 after inoculation. LGR5 (green) and E-cadherin (red). Scale bar = 100 μm. d Histogram of LGR5 signal profile for individual samples at day 35 (left). Intensity of signal per 100 μm distance from tumor interface to center (0–500 μm) (right).

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