Fig. 4: Formation of sCL from glands is reproduced in a 3D culture model using co-culturing system with fibroblasts as feeder cells. | Laboratory Investigation

Fig. 4: Formation of sCL from glands is reproduced in a 3D culture model using co-culturing system with fibroblasts as feeder cells.

From: Continuous formation of small clusters with LGR5-positive cells contributes to tumor growth in a colorectal cancer xenograft model

Fig. 4

a Schematic of the culture system. Scale bar = 50 μm. b Quantitative analysis for formation of sCL-like structures from main ductal structure. c Cancer organoid with feeder cells in time course. Rectangles indicate sCL-like structures detaching from main cancer organoid. Scale bar = 500 μm. d Expression of LGR5 (red), Ki67 (green), and phalloidin (white) in sCL-like structures. Scale bars = 200 μm (low) and 30 μm (high).

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