Fig. 1: The newborn pig gallbladder epithelium expresses apical CFTR. | Laboratory Investigation

Fig. 1: The newborn pig gallbladder epithelium expresses apical CFTR.

From: Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model

Fig. 1

a qRT-PCR-determined CFTR expression in newborn non-CF pig tissue samples. Data are relative to tracheal CFTR expression. Each symbol represents a different animal. The y-axis is the fold change calculated using the following-equation: fold change = 2−ΔΔCT where ΔCT is the difference between the CT value for CFTR and the housekeeping gene, β-actin, and ΔΔCT represents the difference between the ΔCT values for the tissue of interest normalized to tracheal expression. b, c Apical CFTR in newborn gallbladder detected by immunohistochemistry (brown, b) and immunocytochemistry (green, c) for non-CF and CF, top and bottom, respectively, (scale bar = 10 μm).

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