Fig. 2: FOXF1 expression after acid and acidic bile treatment. | Laboratory Investigation

Fig. 2: FOXF1 expression after acid and acidic bile treatment.

From: Forkhead box F1 induces columnar phenotype and epithelial-to-mesenchymal transition in esophageal squamous cells to initiate Barrett’s like metaplasia

Fig. 2

Four normal esophageal squamous cell lines, EPC2, Het-1A, NESB10, and NESG4 were studied. At 70–80% confluency cell lines were exposed to acid at pH 5.5 and/or acidic bile (bile salts @100 μM at pH 5.5.). In A, FOXF1 was evaluated by immunocytochemistry (magnification ×20; scale bar = 24 µm) or immunofluorescence (scale bar = 125 µm) after being treated with acid and acidic bile for 30 min. In B, extracts of nuclear proteins were blotted against FOXF1 after treatment with acidic bile for duration varying from 5 to 30 min.

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