Fig. 3: Dose-dependent effects of FOXF1 on squamous and columnar cytokeratins in normal esophageal squamous EPC2 cells.

At 70% confluency, EPC2 cells were transfected with FOXF1 at varying doses (0.0004–4 µg) or empty vector and columnar cytokeratins 18 (A) and 7 (B) and squamous cytokeratins 10 (C) and 13 (D) were measured by qPCR. Data represent mean ± SEM of four independent experiments.