Fig. 6: FOXF1 overexpression induced EMT and promoted cell motility in normal esophageal squamous cells. | Laboratory Investigation

Fig. 6: FOXF1 overexpression induced EMT and promoted cell motility in normal esophageal squamous cells.

From: Forkhead box F1 induces columnar phenotype and epithelial-to-mesenchymal transition in esophageal squamous cells to initiate Barrett’s like metaplasia

Fig. 6

EPC2 cells at 70% confluency were transfected with FOXF1 (0.0004 µg) or empty vector. A E-cadherin, Snail, and vimentin were evaluated by immunocytochemistry. B Cell motility assays were performed. Bar graph shows quantification of the wound dimensions using Photoshop arbitrary relative units (see “Materials and methods”), *P < 0.05. Data represent mean ± SEM of three independent experiments. Magnification ×20; scale bar = 24 µm.

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