Fig. 1: EZH2 suppressed FOXO3 expression in asthma in an epigenetic manner. | Laboratory Investigation

Fig. 1: EZH2 suppressed FOXO3 expression in asthma in an epigenetic manner.

From: Potential role for EZH2 in promotion of asthma through suppression of miR-34b transcription by inhibition of FOXO3

Fig. 1

A Potential downstream targets of EZH2 predicted by the bioinformatics databases, RNAInter, StarBase, and POSTAR. B Gene interaction network analyzed by STRING; the circles in the picture represent the degree of the gene from large to small; the circle color from blue to orange indicates the degree from large to small; the line in the middle of the circle represents the interaction between genes. C Venn map of intersection of asthma-related genes and target genes in GeneCards database. D Lung tissue lesions determined by HE staining. E Collagen deposition analyzed by Masson’s trichrome staining. F Inflammatory cytokines and EOS in BALF determined by ELISA. G Expression of EZH2 and FOXO3 determined by western blot analysis. H Enrichment of EZH2 in FOXO3 promoter analyzed by ChIP. I Enrichment of H3K27me3 in the FOXO3 promoter measure by ChIP. J Expression of EZH2 and FOXO3 in mouse airway epithelial cells detected by western blot analysis. K Enrichment of H3K27me3 in the FOXO3 promoter in mouse BECs analyzed by ChIP. *p < 0.05 compared to saline group or sh-NC. Statistical comparisons were performed using unpaired t-test when only two groups were compared or by Tukey’s test-corrected one-way ANOVA with when more than two groups were compared. N = 8.

Back to article page