Fig. 3: FOXO3 directly binds to the miR-34b promoter and enhances its transcription.

A miR-34b determined by RT-qPCR. B Enrichment of FOXO3 in miR-34b promoter analyzed by ChIP. C FOXO3 expression levels determined by western blot analysis. D Expression of miR-34b determined by RT-qPCR. E Enrichment of FOXO3 in miR-34b promoter analyzed by ChIP. *p < 0.05 compared to Saline or oe-NC. #p < 0.05 compared to sh-NC. Statistical comparisons were performed using unpaired t-test when only two groups were compared.