Fig. 4: Overexpression of FOXO3 suppressed inflammation and expression of TGF-β, MMP9, and TIMP-1 by facilitating miR-34b expression.

A FOXO3 expression determined by western blot analysis. B miR-34b determined by RT-qPCR. C Inflammatory cytokines measured by ELISA. D TGF-β, MMP9, and TIMP-1 determined by western blot analysis. *p < 0.05 compared to oe-NC + inhibitor NC. #p < 0.05 compared to oe-FOXO3 + inhibitor NC. Statistical comparisons were performed by Tukey’s test-corrected one-way ANOVA when more than two groups were compared.