Fig. 4: XIST as a ceRNA of miR-214-3p upregulated Arl2. | Laboratory Investigation

Fig. 4: XIST as a ceRNA of miR-214-3p upregulated Arl2.

From: LncRNA XIST shuttled by adipose tissue-derived mesenchymal stem cell-derived extracellular vesicles suppresses myocardial pyroptosis in atrial fibrillation by disrupting miR-214-3p-mediated Arl2 inhibition

Fig. 4: XIST as a ceRNA of miR-214-3p upregulated Arl2.

A StarBase database showed that XIST and miR-214-3p had potential binding sites. B Relative luciferase activity after co-transfection of XIST-WT/MUT and miR-214-3p/NC mimic into HEK293T cells. C TargetScan database predicted that Arl2 and miR-214-3p had potential binding sites. D Relative luciferase activity after co-transfection of Arl2-WT/MUT and miR-214-3p/NC mimic into HEK293T cells. E miR-214-3p expression in HL-1 cells detected by qRT-PCR. F Arl2 expression in HL-1 cells detected by qRT-PCR. G miR-214-3p, XIST, and Arl2 expression in HL-1 cells detected by qRT-PCR. H Arl2 expression in HL-1 cells detected by Western blot analysis. *p < 0.05 versus HEK293T transfected with NC mimic, HL-1 cells, or HL-1 cells receiving AF induction and NC inhibitor; #p < 0.05 versus HL-1 cells receiving AF induction and PBS; &p < 0.05 versus HL-1 cells receiving AF induction and NC EVs. Comparison between two groups was analyzed by unpaired t-test and among multiple groups was by one-way ANOVA with Tukey’s post hoc test. All experiments were repeated three times with similar results.

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