Fig. 7: Effect of ASIC1a on the proliferation of RASFs through ERK/MAPK signaling.
From: ASIC1a promotes the proliferation of synovial fibroblasts via the ERK/MAPK pathway
A, B Western blotting analysis of p-c-Raf and Raf expressions in RASFs divided into control, pH 6.0, control shRNA, ASIC1a-shRNA, PcTx-1, vector, and OE-ASIC1a groups. C, D Western blotting analysis of p-ERK1/2 and ERK1/2 expressions in RASFs divided into control, pH 6.0, control shRNA, ASIC1a-shRNA, PcTx-1, vector, and OE-ASIC1a groups. E Western blotting analysis of PCNA expression levels in RASFs with pretreatment of acid (pH 6.0) and U0126 (10 μM) for 12 h. β-actin was served as an endogenous control and the results of gray density were analyzed by ImageJ software. F The colony formation assay was used to analyze the proliferation ability of RASFs with acid (pH 6.0) and U0126 (10 μM) for 12 h. G CCK-8 analysis of the cell proliferation rate of RASFs after treatment with acid (pH 6.0) and U0126 (10 μM) for 12 h. All data are presented as the mean ± S.E.M. for the three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
