Fig. 2: H2O2 treatment induced cell apoptosis and up-regulated MIR22HG level in TM3 cells. | Laboratory Investigation

Fig. 2: H2O2 treatment induced cell apoptosis and up-regulated MIR22HG level in TM3 cells.

From: Long noncoding RNA MIR22HG promotes Leydig cell apoptosis by acting as a competing endogenous RNA for microRNA-125a-5p that targets N-Myc downstream-regulated gene 2 in late-onset hypogonadism

Fig. 2

Mouse LC cell line TM3 was incubated with different concentrations of H2O2 (100, 200, and 400 μM). a The ratio of apoptotic cells was quantified using flow cytometry. b Cell proliferation was detected using EdU immunofluorescence analysis (Scale bar = 100 µm) and the EdU positive cells (green) were calculated. DAPI (blue) was used to counterstain the cell nucleus. c The concentration of testosterone in cells was determined using ELISA. d The relative expression level of MIR22HG was determined using qRT-RCR. The cells without H2O2 treatment were used as control. *P < 0.05.

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