Table 1 The sequences of primers used in this study were shown as below.

From: Long noncoding RNA MIR22HG promotes Leydig cell apoptosis by acting as a competing endogenous RNA for microRNA-125a-5p that targets N-Myc downstream-regulated gene 2 in late-onset hypogonadism

Genes

Forward primer (5′–3′)

Reverse primer (5′–3′)

β-actin

ACTGCCGCATCCTCTTCCT

TCAACGTCACACTTCATGATGGA

MIR22HG

CCAGTTGAAGAACTGTTGCCC

CGTATCATCCACCCTGCTGT

NDRG2

TCGTGCGGGTTCATGTGGATGC

TGTGTCCGGGTGGTTCAGAGCA

U6

CGCTTCACGAATTTGCGTGTCAT

CGCTTCACGAATTTGCGTGTCAT

miR-125a-5p

TCCCTGAGACCCTTTAACCTGTGA

 

hsa-miR-361-5p

TTATCAGAATCTCCAGGGGTAC

 

hsa-miR-150-5p

TCTCCCAACCCTTGTACCAGTG

 

hsa-miR-133a-3p

TTTGGTCCCCTTCAACCAGCTG

 

hsa-miR-1301-3p

TTGCAGCTGCCTGGGAGTGACTTC

 
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