Fig. 2: Proteomic detection of contaminating proteins in rhMOG batches, and their stimulating effect on innate immunity. | Laboratory Investigation

Fig. 2: Proteomic detection of contaminating proteins in rhMOG batches, and their stimulating effect on innate immunity.

From: Recombinant myelin oligodendrocyte glycoprotein quality modifies evolution of experimental autoimmune encephalitis in macaques

Fig. 2

A The spectral count of E.coli proteins detected in the batches of rhMOG-1 and rhMOG-2 were quantified and normalized according to their molecular weight and expressed as normalized spectral abundance factor (NSAF). Host cell proteins from E. coli of all ranges of molecular weights were much more abundant in rhMOG-1 than in rhMOG-2. B, C TLR2 and TLR4 stimulatory activity of rhMOG-1 and rhMOG-2 as measured in HEK293 cells transfected with TLR2 or TLR4. Positive controls include stimulation with specific ligands of TLR2 (PAM3CSK (PAM) at 1 µg/ml or TNFα at 25 ng/ml) and TLR4 (ULPS at 10 µg/ml or TNFα at 25 ng/ml). Statistics, Two-way ANOVA with Tuckey multiple comparisons test. P value summary, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, ****P ≤ 0.0001.

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