Fig. 2: Physical and biochemical characterization of osteosarcoma exosomes.

A Scanning electron micrographs of OSCA-40 exosomes. B Immunoblotting of exosome preparations documenting enrichment of tetraspanins (CD63) and depletion of β-actin in OSCA-8, OSCA-32, OSCA-40, and OSCA-78 dog osteosarcoma cell lines. C NanoSight particle tracking analysis of triplicate samples each of osteosarcoma cell lines OSCA-8, OSCA-32, OSCA-40, and OSCA-78, showing modal diameters of approximately 130 nm.