Fig. 1: Expression of HULC in glioma tissues and GSCs. | Laboratory Investigation

Fig. 1: Expression of HULC in glioma tissues and GSCs.

From: Induction of cancer cell stemness in glioma through glycolysis and the long noncoding RNA HULC-activated FOXM1/AGR2/HIF-1α axis

Fig. 1: Expression of HULC in glioma tissues and GSCs.

A Expression of HULC in clinical tissue samples determined using RT-qPCR (n = 50 in the GBM group, n = 35 in the control group); *p < 0.05 compared with the control group. B Expression of HULC in the glioma cell lines determined using RT-qPCR; *p < 0.05 compared with the HEB group. C Sorting of CD133 + GSCs using flow cytometry. D Morphological observation of the spheroids that formed from cultured GSCs in vitro. E Expression of CD133 and Nestin in the GSC spheres determined using immunofluorescence staining. F Expression of the GSC marker genes (OCT4, SOX2, and Nanog) determined using RT-qPCR; *p < 0.05 compared with the U251 group. G Expression of HULC in GSCs determined using RT-qPCR; *p < 0.05 compared with the U251 group. All experiments were repeated three times.

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