Fig. 1: Analysis of YAP/TAZ activation and nuclear translocation. | Laboratory Investigation

Fig. 1: Analysis of YAP/TAZ activation and nuclear translocation.

From: Mechanical loading activates the YAP/TAZ pathway and chemokine expression in the MLO-Y4 osteocyte-like cell line

Fig. 1

A E11/gp38 and Ptsg2 (encoding COX-2) gene expression assessed by real-time qPCR in response to mechanical loading in 2D (stretching) or 3D culture (compression) (B) YAP/TAZ target genes expression assessed by real-time qPCR after mechanical loading (C) YAP/TAZ nuclear translocation measured from Apotome immunofluorescence images of YAP/TAZ (in green) and nuclei (DAPI, in blue). Scale bars: 20 µm. Graphic represent the percentage of nuclear YAP/TAZ positive cells. Each dot plot represents individual collagen hydrogel obtained from three different experiments. D YAP1 and WWTR1 (TAZ) gene expression assessed by real-time qPCR in response to mechanical loading (E) Immunoblotting of YAP and TAZ from MLO-Y4 submitted to mechanical loading in 3D culture. TAZ immunoblotting was performed once. YAP quantification involved three different experiments. Data are mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 by Mann–Whitney test or two-way ANOVA.

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