Table 1 Primer sequences for real-time PCR analyses.
Gene | GenBank | Primer sequence, 5′ to 3′ | Product size (bp) | Annealing temperature |
|---|---|---|---|---|
GAPDH | NM_001289745 | F: AACGGATTTGGTCGTATTGCA | 215 | 60 °C |
R: CGCTCCTGGAAGATGGTGAT | ||||
FABP4 | NM_001442.3 | F: CAGGAAAGTGGCTGGCATGGC | 293 | 60.3 °C |
R:CTCTCGTGGAAGTGACGCCTTTC | ||||
JAK2 | NM_001322194 | F: TTATTCAGCAATTCAGCCAATG | 215 | 58.2 °C |
R: TCACTTTCTTTATGTTTCCCTCTT | ||||
STAT2 | NM_198332 | F: ATGGGCTTTGTGAGTCGG | 170 | 56.5 °C |
R: GCCTTCGTGTACGGTTGC | ||||
SOCS1 | NM_003745 | F: CGACACGCACTTCCGCACATT | 158 | 58.5 °C |
R: GGGTCCCGAGGCCATCTTCAC | ||||
Rap1a | NM_001010935.3 | F: CGTGAGTACAAGCTAGTGGTCC | 166 | 61.4 °C |
R: CCAGGATTTCGAGCATACACTG | ||||
CCL8 | NM_005623 | F:TGGAGAGCTACACAAGAATCACC | 133 | 61.4 °C |
R:TGGTCCAGATGCTTCATGGAA | ||||
CXCL10 | NM_001565 | F:GTGGCATTCAAGGAGTACCTC | 198 | 60.8 °C |
R:TGATGGCCTTCGATTCTGGATT | ||||
IL-1β | NM_000576.3 | F: GCGGCATCCAGCTACGAATCTC | 244 | 55.3 °C |
R: CGGAGCGTGCAGTTCAGTGATC | ||||
IL-6 | NM_000600.5 | F: CTCACCTCTTCAGAACGAATTG | 149 | 60.8 °C |
R: CCATCTTTGGAAGGTTCAGGTTG | ||||
TNF-α | NM_000594.4 | F: CCTCTCTCTAATCAGCCCTCTG R: GAGGACCTGGGAGTAGATGAG | 220 | 60.5 °C |
