Fig. 4: 2D cultures of human osteosarcoma cell lines are sensitive to CDK4/CDK6 inhibitor palbociclib. | Laboratory Investigation

Fig. 4: 2D cultures of human osteosarcoma cell lines are sensitive to CDK4/CDK6 inhibitor palbociclib.

From: A murine mesenchymal stem cell model for initiating events in osteosarcomagenesis points to CDK4/CDK6 inhibition as a therapeutic target

Fig. 4

A Osteosarcoma cell lines were treated with palbociclib for 72 h after which relative cell viability and IC50 values were determined. Cell lines MG63 and 143BHOS (green) have loss of p16, whereas other OS cell lines have intact p16 (blue). Data points represent the mean of three experiments performed ± standard deviation. B Western blot showing expression levels of Rb in osteosarcoma cell lines. GAPDH was used as a loading control. C p16 protein expression status of each osteosarcoma cell line as published21 was correlated with IC50 values from the current study. n.s. not statistically significant. D CDK4 and CDK6 RNA expression levels as published elsewhere30 of each cell line did not correlate with IC50 values from the current study. Each dot represents one cell line.

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