Fig. 5: 3D cultured MCTS of osteosarcoma cell lines are also sensitive to palbociclib. | Laboratory Investigation

Fig. 5: 3D cultured MCTS of osteosarcoma cell lines are also sensitive to palbociclib.

From: A murine mesenchymal stem cell model for initiating events in osteosarcomagenesis points to CDK4/CDK6 inhibition as a therapeutic target

Fig. 5

A Hematoxylin and eosin staining of osteosarcoma MCTS treated with palbociclib for 72 h. Scalebar represents 20 µm. Inset shows SATB2 staining. B Relative cell viability and IC50 values were determined after treatment with palbociclib. C Cleaved caspase 3 and Ki67 staining and quantification of 3D cultured MCTS of OS cell lines treated with 10 µM palbociclib (Palb) or PBS. Scale bar represents 20 µm. n.s. not statistically significant, **p ≤ 0.01, ***p ≤ 0.001.

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