Fig. 2: Generation of PKD1insG/+ cloned pigs by somatic cell nuclear transfer.
From: Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation
A Cloned blastocysts generated using a PKD1insG/+ nuclear donor cell (clone #132), which were then transferred into recipient gilts. B Genotyping of four live PKD1insG/+ cloned pigs (K91, K92, K93, and K94) and one stillborn (No. 51) by PCR-RFLP analysis. One or three bands were obtained after BsrI digestion of the PCR amplicon from WT pigs and PKD1insG/+ pigs, respectively. Arrowheads indicate the digested fragments from the PKD1insG mutant allele with a 1-bp insertion of a guanine nucleotide. M DNA Ladder, N non-digested, d BsrI digested. C Photograph of a stillborn PKD1insG/+ cloned pig (No. 51). The bar represents 3 cm. D Gross morphology of a kidney from the stillborn piglet. Yellow arrowheads indicate renal cysts. E, F Histological section of the kidney (Masson’s trichrome staining). Asterisks indicate a renal cyst. Bars = 1 mm (E) and 100 µm (F).
