Fig. 3: Phenotypes of PKD1insG/+ founder cloned pigs.
From: Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation
A Ultrasound diagnostic images of the kidney from a PKD1insG/+ cloned pig (K94) at 5 months of age. Gross morphology (B) and coronal section (C) of a kidney from a PKD1insG/+ cloned pig. The yellow bar represents 3 cm. Yellow arrowheads indicate renal cysts. D Relative PKD1 expression in three PKD1insG/+ cloned pigs evaluated using quantitative RT-PCR. The data are presented as the mean ± SD. *p < 0.05. E, F Histological analysis of a PKD1insG/+ founder cloned pig at 5 months of age. Kidney tissue sections with Masson’s trichrome staining (E) and immunostaining using a PKD1-specific antibody (F). Asterisks indicate renal cysts. The black bars represent 250 µm in the left panels and 100 µm in the right panels.
