Fig. 1: USP35 interacts with BIRC3. | Laboratory Investigation

Fig. 1: USP35 interacts with BIRC3.

From: Ubiquitin-specific protease 35 (USP35) mediates cisplatin-induced apoptosis by stabilizing BIRC3 in non-small cell lung cancer

Fig. 1

A H460 cells were transiently transfected with the Myc vector or Myc-USP35 expression plasmid for 48 h. Cell lysates were purified, separated on gels and then stained with Coomassie blue R-250. A specific protein band was evaluated using mass spectrometric analysis. B HEK293T cells were transiently cotransfected with Myc-USP35 and Flag-BIRC3 or Flag-tagged control plasmids, and cell lysates were immunoprecipitated with an anti-Flag antibody followed by immunoblotting with an anti-Myc antibody. C HEK293T cells were transiently cotransfected with Flag-BIRC3 and Myc-USP35 or Myc-tagged control plasmids, and cell lysates were immunoprecipitated with an anti-Myc antibody followed by immunoblotting with an anti-Flag antibody. D Lysates of Anip973 cells were immunoprecipitated with an anti-USP35 or IgG antibody, followed by immunoblotting with an anti-BIRC3 antibody. E Lysates of Anip973 cells were immunoprecipitated with an anti-BIRC3 or IgG antibody, followed by immunoblotting with anti-USP35 antibody. All results are representative of three independent experiments.

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