Fig. 4: MFF-SIRT1/3 axis was regulated by miR-34-5p. | Laboratory Investigation

Fig. 4: MFF-SIRT1/3 axis was regulated by miR-34-5p.

From: The MFF-SIRT1/3 axis, regulated by miR-340-5p, restores mitochondrial homeostasis of hypoxia-induced pulmonary artery smooth muscle cells

Fig. 4

A The expression of miR-340-5p in hypoxia-treated PAMSCs was determined using qRT-PCR. B Targetscan software was applied to predict the binding site between miR-340-5p and MFF. C The transfection of miR-340-5p mimics was measured by qRT-PCR assay. D PAMSCs were cultured and co-transfected with MFF-wt or MFF-mut plasmids and miR-340-5p mimics or mimics NC, then cell luciferase activity was measured. E The mRNA level of MFF in miR-340-5p overexpressed PAMSCs was detected by qRT-PCR. F The protein levels of MFF, SIRT1 and SIRT3 were detected by western blot. The data were expressed as mean ± SD. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001.

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