Fig. 5: miR-340-5p protected mitochondrial function and proliferation–apoptosis balance in hypoxia-treated PAMSCs via downregulating MFF. | Laboratory Investigation

Fig. 5: miR-340-5p protected mitochondrial function and proliferation–apoptosis balance in hypoxia-treated PAMSCs via downregulating MFF.

From: The MFF-SIRT1/3 axis, regulated by miR-340-5p, restores mitochondrial homeostasis of hypoxia-induced pulmonary artery smooth muscle cells

Fig. 5

Hypoxia-treated PAMSCs were transfected with miR-340-5p mimics or co-transfected with pcDNA-MFF and miR-340-5p mimics. A, B The expression of miR-340-5p and MFF in hypoxia-treated PAMSCs were assessed using qRT-PCR. C MTT assay was employed to determine cell viability of PAMSCs. D The level of ROS in PAMSCs was assessed using flow cytometry. E The mitochondrial membrane potential was evaluated using JC-1 assay. F The content of ATP in PAMSCs was detected using kit. G The mitochondrial network structure in PAMSCs was examined by Mito Tracker Red staining, and the mitochondrial perimeter was determined. H Cell apoptosis of PAMSCs was evaluated using TUNEL staining. I The caspase-3 activity in PAMSCs was assessed using kit. The data were expressed as mean ± SD. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001.

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