Fig. 4: CREB1 regulated SRGN expression by targeting its promoter. | Laboratory Investigation

Fig. 4: CREB1 regulated SRGN expression by targeting its promoter.

From: STAT3 regulates SRGN and promotes metastasis of nasopharyngeal carcinoma through the FoxO1-miR-148a-5p-CREB1 axis

Fig. 4

A Western blot analysis of CREB1 and SRGN in 5-8F cells transfected with NC and CREB1. B The targeting site of CREB1 at the promoter region of SRGN was predicted by JASPAR analysis. C EMSA was performed to validate the interplay between the CREB1 protein and SRGN promoter in 5-8F cells. D ChIP-PCR was employed to assess CREB1 binding to the SRGN promoter in CREB1-silenced 5-8F cells. All the results are shown as the mean ± SD (n = 3), and three different experiments were performed in triplicate. *P < 0.05, **P < 0.01.

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