Fig. 6: FoxO1 silencing facilitated NPC tumor progression in vitro. | Laboratory Investigation

Fig. 6: FoxO1 silencing facilitated NPC tumor progression in vitro.

From: STAT3 regulates SRGN and promotes metastasis of nasopharyngeal carcinoma through the FoxO1-miR-148a-5p-CREB1 axis

Fig. 6

A The knockdown efficiency of FoxO1 protein was verified by western blotting. B The proliferation of HONE1 and 5-8F cells was analyzed by the CCK-8 assay after 0, 1, 2, and 3 days of FoxO1 shRNA transfection. C The effects of FoxO1 knockdown on the colony formation ability of HONE1 and 5-8F cells were assessed by the colony formation assay. D Wound healing and E Transwell assays were used to analyze the migratory and invasive capacities of HONE1 and 5-8F cells after FoxO1 knockdown, respectively. F Protein expression of CREB1 and SRGN was detected in tumors formed by FoxO1-silenced HONE1 and 5-8F cells by western blotting. G Relative miR-148a-5p expression was evaluated by qRT-PCR in tumors formed by FoxO1-silenced HONE1 and 5-8F cells. All the results are shown as the mean ± SD (n = 4), and three different experiments were performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001.

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