Fig. 7: FoxO1 binds to the promoter of MIR148, while miR-148a-5p targets CREB1. | Laboratory Investigation

Fig. 7: FoxO1 binds to the promoter of MIR148, while miR-148a-5p targets CREB1.

From: STAT3 regulates SRGN and promotes metastasis of nasopharyngeal carcinoma through the FoxO1-miR-148a-5p-CREB1 axis

Fig. 7

A The binding sites between miR-148a-5p and CREB1 were predicted by starBase software. B A dual-luciferase reporter assay was conducted to validate the interplay between miR-148a-5p and CREB1 using two individual binding sites predicted by starBase software. C The RIP assay was performed to further confirm the interplay between miR-148a-5p and CREB1. D qRT-PCR was carried out to detect the expression of primary and mature forms of miR-148a-5p after FoxO1 overexpression. E The targeting site of FoxO1 at the promoter region of MIR148 was predicted by JASPAR analysis. F ChIP assays were conducted to verify the binding between FoxO1 and MIR148. G The interplay between MIR148 and FoxO1 was verified by a dual-luciferase reporter assay. All the results are shown as the mean ± SD (n = 3), and three different experiments were performed in triplicate. **P < 0.01, ***P < 0.001. ns no significant.

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