Fig. 8: STAT3 silencing repressed NPC tumor progression and regulated CREB1 and SRGN through FoxO1 in vitro. | Laboratory Investigation

Fig. 8: STAT3 silencing repressed NPC tumor progression and regulated CREB1 and SRGN through FoxO1 in vitro.

From: STAT3 regulates SRGN and promotes metastasis of nasopharyngeal carcinoma through the FoxO1-miR-148a-5p-CREB1 axis

Fig. 8

A Knockdown efficiency of STAT3 protein by shRNAs was examined by western blotting. B, C CCK-8 and colony formation assays were utilized to detect cell proliferation after STAT3 knockdown. D, E Wound healing and Transwell assays were performed to analyze cell migration and invasion after STAT3 knockdown. F Protein levels of FoxO1, CREB1 and SRGN were detected by western blotting in STAT3-silenced cells. G Relative expression of miR-148a-5p was examined in cells transfected with shNC and shSTAT3. H Protein levels of STAT3, FoxO1, CREB1 and SRGN were detected by western blotting in STAT3- and/or FoxO1-silenced cells. I The relative expression of miR-148a-5p was examined in cells transfected with shSTAT3 and/or shFoxO1. All the results are shown as the mean ± SD (n = 3), and three different experiments were performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001.

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