Fig. 1: Nesfatin-1 attenuated acidosis-induced cytotoxicity in primary rat articular chondrocyte. | Laboratory Investigation

Fig. 1: Nesfatin-1 attenuated acidosis-induced cytotoxicity in primary rat articular chondrocyte.

From: Nesfatin-1 exerts protective effects on acidosis-stimulated chondrocytes and rats with adjuvant-induced arthritis by inhibiting ASIC1a expression

Fig. 1

A Nesfatin-1 restored cell viability in acidosis-induced articular chondrocytes, which was determined using the MTT assay. B Cell viability was assessed with the MTT assay in acid-stimulated chondrocytes after nesfatin-1 and PcTx1 pretreatment. C Membrane ASIC1a protein was detected by western blotting in articular chondrocytes, with or without nesfatin-1 treatment. D Representative phase-contrast images showing chondrocytes, which were obtained after treatment with the indicated solutions. Data represent the mean ± SEM values for three independent experiments. **P < 0.01 compared to the control group; #P < 0.05, ##P < 0.01 compared to the pH 6.0 group; ns: no significance.

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