Fig. 5: Nesfatin-1 attenuated acid-induced oxidative stress, inflammation, and apoptosis in articular chondrocytes. | Laboratory Investigation

Fig. 5: Nesfatin-1 attenuated acid-induced oxidative stress, inflammation, and apoptosis in articular chondrocytes.

From: Nesfatin-1 exerts protective effects on acidosis-stimulated chondrocytes and rats with adjuvant-induced arthritis by inhibiting ASIC1a expression

Fig. 5

A 2’,7’-dichlorofluorescin (DCF) staining of intracellular ROS. Nesfatin-1 reduced acid-induced ROS generation. B Effects of nesfatin-1 on acid-induced MDA levels. C Effects of nesfatin-1 on acid-induced GSH levels. D–F The mRNA levels of inflammatory cytokines (IL-6, IL-1β, and TNF-α) in acid-mediated chondrocytes after nesfatin-1 and PcTx1 pretreatment were measured using real time-PCR. G The IL-6, IL-1β, and TNF-α protein levels in acid-mediated chondrocytes after nesfatin-1 and PcTx1 pretreatment were measured using western blotting. H–J The IL-6, IL-1β, and TNF-α levels in the culture supernatant after nesfatin-1 and PcTx1 pretreatment were measured using ELISA. K Acid-induced chondrocyte apoptosis after nesfatin-1 and PcTx1 pretreatment was detected using the Annexin-V/PI assay. L, M Acid-induced chondrocyte apoptosis after nesfatin-1 and PcTx1 pretreatment was detected using the TUNEL assay. N Bax, Bcl-2, cleaved caspase-9/caspase-9, and cleaved-PARP/PARP protein levels were analyzed by western blotting. Data represent the mean ± SEM values for three independent experiments. **P < 0.01 compared to the control group; #P < 0.05, ##P < 0.01 compared to the pH 6.0 group; ns: no significance.

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