Fig. 3: Concordance of Mean Pixel Intensity by MIBI-TOF. | Laboratory Investigation

Fig. 3: Concordance of Mean Pixel Intensity by MIBI-TOF.

From: Reproducible, high-dimensional imaging in archival human tissue by multiplexed ion beam imaging by time-of-flight (MIBI-TOF)

Fig. 3

A Representative MIBI-TOF images from serial sections of the same TMA core of lymph node tissue. B Plot of the average Mean Pixel Intensity (MPI) of all FOVs of the same TMA core vs. the MPI of each FOV. Each color represents a different marker. The gray line is a reference line with slope 1 and intercept 0. We performed least squares linear regression of the average MPI of the core vs. the MPI of all six serial sections and found the slope m (C) and coefficient of determination R2 (D) for all 16 markers.

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