Fig. 4: Reproducibility of cell phenotyping by MIBI-TOF. | Laboratory Investigation

Fig. 4: Reproducibility of cell phenotyping by MIBI-TOF.

From: Reproducible, high-dimensional imaging in archival human tissue by multiplexed ion beam imaging by time-of-flight (MIBI-TOF)

Fig. 4

A Cells were assigned to a phenotype using an unsupervised clustering approach and manually annotated into eight major cell types. Expression values were z-scored for each marker. High z-scores are an indicator of high marker specificity. B The Spearman correlation between all serial sections of each TMA core using the frequency of cell types in each FOV. C Representative images of six serial sections of the same TMA core of tonsil tissue. Top row: Cell phenotype map colored according to the eight cell types shown in (A). Middle and bottom rows: MIBI-TOF images showing CD20 and PanCK expression.

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