Fig. 3: LRF/OCZF protein was detected in osteoclasts expressing cathepsin K at bone destruction sites of adjuvant-induced arthritis rats.

Immunofluorescence staining of osteoclasts in bone destruction sites of arthritic rats. Cryosections of ankle joints were incubated with rabbit anti-OCZF and mouse anti-cathepsin K antibodies, followed by detection with secondary antibodies. Arrow indicates typical osteoclasts containing three or more nuclei on bone. LRF/OCZF, green; cathepsin K, red; bone marrow, BM; Bone, B; scale bar = 50 μm.