Fig. 6: Expression of SAM68 protein was markedly decreased in OCZF-Tg mice-derived osteoclasts. | Laboratory Investigation

Fig. 6: Expression of SAM68 protein was markedly decreased in OCZF-Tg mice-derived osteoclasts.

From: Leukemia/lymphoma-related factor (LRF) or osteoclast zinc finger protein (OCZF) overexpression promotes osteoclast survival by increasing Bcl-xl mRNA: A novel regulatory mechanism mediated by the RNA binding protein SAM68

Fig. 6

A RT-qPCR analysis for the expression of Sam68 mRNA in osteoclastogenesis. BMMs cultured with M-CSF (10 ng/ml) and RANKL (50 ng/ml) were harvested at 0, 36, or 72 h, and subjected to RT-qPCR. Expression levels were normalized to that of Gapdh (n = 3). B Western blotting analysis of SAM68 at 72 h of culture derived from WT and OCZF-Tg BMMs. Cell lysates of osteoclasts from WT and OCZF-Tg mice were analyzed. C Immunostaining of osteoclasts derived from WT and OCZF-Tg mice. SAM68 (red), Nuclei (blue). TRAP staining was performed after immunostaining. D SAM68 protein expression is shown as the average of fluorescence intensity of osteoclasts (n = 10). Data are presented as means ± SD. The differences between means were determined by one-way ANOVA followed by Tukey’s post hoc test in A. **P < 0.01, *P < 0.05. Student’s t test was used for statistical analysis in (D). **P < 0.01, compared to the cells of WT mice.

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