Fig. 2: WISP-2 mRNA and protein expression in human chondrocytes.
From: WISP-2 modulates the induction of inflammatory mediators and cartilage catabolism in chondrocytes
A Human T/C-28a2 cells were treated with TNF-α 0.1 and 1 ng/mL, B IL-1β 0.025, 0.1 and 0.5 ng/mL, and C with leptin (800 nM), adiponectin (10 µg/mL) and visfatin (500 ng/mL) for 24 h. D WISP-2 mRNA basal expression in healthy and OA human primary chondrocytes. Values are the mean ± SEM of 4 independent human samples for the healthy cartilage, and 8 independent human samples for OA cartilage (*p < 0.05). E Immunocytochemical images showing WISP-2 protein expression in healthy and OA human cartilage. F Human primary OA chondrocytes were treated with TNF-α 1 ng/mL, IL-1β 0.1 ng/mL, LPS 250 ng/mL and IL-6 10 ng/mL for 24 h. WISP-2 mRNA expression was evaluated by RT-qPCR. Values are the mean ± SEM of at least three independent experiments (**p < 0.01, ***p < 0.001 vs. control).
