Fig. 6: Effect of WISP-2 siRNA knockdown on IL-1β-stimulated chondrocytes.
From: WISP-2 modulates the induction of inflammatory mediators and cartilage catabolism in chondrocytes
Chondrocytes were transfected with negative control (siC-, 10 nM) or siRNA against WISP-2 (siWISP-2, 10 nM) in presence or not of IL-1β (0.5 ng/mL) for 24 h. A–D Relative mRNA levels of MMP-13, ADAMTS-5, IL-6, and IL-8 in human T/C-28a2 chondrocytes were determined by RT-qPCR. E Determination of human MMP-13 protein expression by western blot in human primary OA chondrocytes. GAPDH was used as a loading control. Densitometric analysis was also shown. Values are the mean ± SEM of at least three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 vs. siC-).
