Fig. 4: Effect of SOX2 on the role of PAK2 in the malignant phenotypes of LSCC cells. | Laboratory Investigation

Fig. 4: Effect of SOX2 on the role of PAK2 in the malignant phenotypes of LSCC cells.

From: p21-activated kinase 2 binds to transcription factor SOX2 and up-regulates DEK to promote the progression of lung squamous cell carcinoma

Fig. 4

A qRT-PCR and Western blot assay to measure the expression of PAK2 and SOX2 in response to oe-PAK2 alone or combined with sh-SOX2; B CCK-8 assay to measure the proliferative ability of NCI-H2170 and NCI-H520 cells in response to oe-PAK2 alone or combined with sh-SOX2; C Transwell assay to detect the migratory and invasive potential of NCI-H2170 and NCI-H520 cells in response to oe-PAK2 alone or combined with sh-SOX2; D Western blot to determine the expression of Ki67, MMP-9, E-cadherin, and N-cadherin in NCI-H2170 and NCI-H520 cells in response to oe-PAK2 alone or combined with sh-SOX2. Measurement data were summarized as mean ± standard deviation. One-way with Tukey’s post hoc test was adopted for comparison among data of multiple groups (for A, C, D). Two-way ANOVA with Tukey’s post hoc test was used for comparison among data at different time points (for B). *p < 0.05 versus the oe-NC + sh-NC group; #p < 0.05 versus the oe-PAK2 + sh-NC. Cell experiment was repeated three times.

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