Fig. 1: Correlation between the expression of Wilms tumor 1-associating protein (WTAP) and regulatory T (Treg) cell proportion in peripheral blood CD4+ T cells of tolerant kidney transplant recipients (TOL).

A The expression profiles of 10 transcripts with the most significant changes in GSE22229. Rows represent transcripts and columns represent blood samples from TOL (n = 19), kidney transplant recipients receiving standard immunotherapy (SI, n = 26), and healthy control subjects (control, n = 13). The color scale runs from blue (low intensity) to white (medium intensity), to red (strong intensity). B–D TOL (n = 20), IR (n = 20), and healthy control (HC; n = 20) were enrolled and the whole-blood samples were collected. CD4⁺ T cells were isolated from the whole-blood samples using dynabeads. B The population of CD25⁺ forkhead box P3 (Foxp3)⁺ cells in CD4⁺ T cells was measured by flow cytometry. C The N6-methyladenosine (m⁶A) concentration in CD4⁺ T cells was measured using an assay kit. D The relative mRNA levels of methyltransferase-like 3 (METTL3), METTL14, WTAP, fat mass and obesity-associated protein (FTO), and alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 (ALKBH5) were measured using qRT-PCR. E Pearson correlation coefficient was used to determine the correlation between METTL3/METTL14/WTAP/ FTO/ALKBH5 and the proportion of CD4⁺CD25⁺Foxp3⁺ cells in the TOL group. ** P < 0.01 vs. HC; ## P < 0.01 vs. IR.