Fig. 6: CircST6GALNAC6/HSPB1 axis regulated bladder cancer cell ferroptosis sensitivity through the p38 MAPK pathway. | Laboratory Investigation

Fig. 6: CircST6GALNAC6/HSPB1 axis regulated bladder cancer cell ferroptosis sensitivity through the p38 MAPK pathway.

From: CircRNA-ST6GALNAC6 increases the sensitivity of bladder cancer cells to erastin-induced ferroptosis by regulating the HSPB1/P38 axis

Fig. 6: CircST6GALNAC6/HSPB1 axis regulated bladder cancer cell ferroptosis sensitivity through the p38 MAPK pathway.

A Protein levels of p38, p-p38, JNK and p-JNK were detected in circST6GALNAC6 over-expressed cells treated with HSPB1-OE in the presence of erastin. B CCK-8 analysis of cell viability in circST6GALNAC6 over-expressed cells treated with HSPB1-OE in the presence of erastin with or without the p38 inhibitor. C ROS level in circST6GALNAC6 over-expressed cells treated with HSPB1-OE in the presence of erastin with or without the p38 inhibitor. D, E qRT-PCR and Western blot analysis of SLC7A11, GPX4 and COX2 in circST6GALNAC6 over-expressed cells in the presence of erastin after treatment with HSPB1-OE with or without the p38 inhibitor. F, G Production of MDA and GSH in circST6GALNAC6 over-expressed cells in the presence of erastin after treatment with HSPB1-OE with or without the p38 inhibitor. H Intracellular iron level in treated cells was determined by fluorescent indicator PGSK. The mean ± SD in the graph shows the relative levels from three replicates. *p < 0.05, **p < 0.01.

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