Fig. 5: Smo-i treatment augments the proliferation of CD8+ T cells in the colon and the mesenteric lymph nodes. | Laboratory Investigation

Fig. 5: Smo-i treatment augments the proliferation of CD8+ T cells in the colon and the mesenteric lymph nodes.

From: Hedgehog blockade remodels the gut microbiota and the intestinal effector CD8+ T cells in a mouse model of mammary carcinoma

Fig. 5

A Flow cytometry was used to assess the abundance and proliferation of conventional CD8+ T cell populations as well as their proliferative status (as assessed by Ki-67 expression) from IELs and B LPLs isolated from vehicle control and Smo-i-treated mice. C The mLNs of vehicle control and Smo-i-treated tumor-bearing mice were excised and analyzed through flow cytometry. The abundance of CD8+ T cells was observed. Each point represents an individual sample for all flow analyses. D Colonic tissue from vehicle control and Smo-i-treated tumor-bearing mice were stained with DAPI (blue) and CD8α (red) by secondary (indirect) IF. Graph represents mean intensity of CD8α+. Each point represents an object of intensity determined by the NIS Elements software. Error bars represents minimum/maximum values. *p < 0.05, **p < 0.005, ***p < 0.001.

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