Fig. 6: USP3 affects the malignant phenotypes and glycolysis of GBC cells by the regulation of PKLR. | Laboratory Investigation

Fig. 6: USP3 affects the malignant phenotypes and glycolysis of GBC cells by the regulation of PKLR.

From: Ubiquitin-specific protease 3 facilitates cell proliferation by deubiquitinating pyruvate kinase L/R in gallbladder cancer

Fig. 6: USP3 affects the malignant phenotypes and glycolysis of GBC cells by the regulation of PKLR.

PKLR was silenced in USP3-overexpressed GBC-SD cells utilizing siRNA. Cell proliferation productivity of GBC-SD cells transfected with OE-USP3 and siRNA-PKLR-1/2 was assessed by CCK8 assay (A). The cellular glucose consumption (B) and lactic acid content (C) were further evaluated. Data are expressed as mean ± SD. ns no significant, ※, p < 0.05; ※※, p < 0.01.

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