Fig. 5

Overexpression of PI3Kγ subunits affects the migration of malignant B cells. a Confirmation of p110γ-GFP and p101-GFP overexpression in JVM3 cells. JVM3 cells were transfected with either p101-GFP or p110γ-GFP plasmids. 24 h post transfection, RNA was harvested and expression of p101 or p110γ was determined by RT-qPCR analysis. b Overexpression of p110γ or p101 enhances the migration of JVM3 in response to the chemokine SDF1α. 24 h after transfection with p101-GFP plasmid or p110γ-GFP, migration of live GFP-expressing cells in response to SDF1α (100 ng/ml) was assessed using a transwell migration assay. The graph shows the mean and SEM of four experiments. c Overexpression of PI3Kγ subunits enhances CLL cell migration. p110γ-GFP, p101-GFP, or both together were overexpressed in CLL cells. After 48 h, migration of live GFP+ cells was assessed by transwell assays as above. Results are expressed as fold change in migration relative to the control transfection of the same patient sample