Fig. 7

PI3Kδ and PI3Kγ inhibitors differentially affect cytoskeletal remodeling and cell morphology. a Time-lapse images of CZC24832-treated Ramos cells migrating within SDF1α gradient, showing the dynamic formation and retraction of multiple protrusions and failure to form a stable polarized morphology. Note that all three cells in this field exhibit multiple protrusions at one of the time points (indicated by arrows) and failed to migrate significantly in the direction of the SDF1α gradient. b Morphological scoring demonstrating the differential impact of PI3Kδ and PI3Kγ inhibitors on Ramos cell polarization. Ramos cells were pretreated with inhibitors, stimulated with 100 ng/ml SDF1α for 1 min, then fixed and F-actin stained using Alexa-488 phalloidin. Representative CZC24832-treated cells exhibiting the four major observed morphologies are shown. c Frequency of cells exhibiting each morphology within control and inhibitor-treated groups. Data are based on three independent experiments scoring over 200 cells per treatment group in total. Paired t-test, asterisk denotes significance comparing drug-treated to control, plus denotes significance comparing CZC24832 to Idelalisib