Fig. 3

Overview of large-scale vector manufacturing. Manufacture of a lentiviral vector begins with the culture of a packaging cell line in a facility that uses Good Manufacturing Practices. The cells are transfected with the plasmids that make up the third-generation lentiviral vector, and the vector-producing cells are expanded in culture. The vector is purified from the cells and culture debris and filtered to ensure sterility, and individual aliquots are cryopreserved