Fig. 1

Pre-B leukemia impairs hematopoiesis in the bone marrow. a Schematic representation of the experimental model. The normal bone marrow cells were transduced with MSCV-BCR-ABL1-IRES-mCherry retrovirus and transplanted into lethally irradiated Recipient 1. All subsequent transplantations were performed using non-irradiated recipients. b Kaplan–Meier survival curve of mice transplanted with 1000 BCR-ABL1 ALL cells (n = 24 mice). c Percentage of mCherry⁺ cells in bone marrow, spleen and blood during leukemia development. d Number of hematopoietic cells (CD45⁺mCherry⁻) in the bone marrow during leukemia development. e Percentage of B220⁺ cells in the CD45⁺mCherry⁻ fraction during leukemia development. f Percentage of CD11b⁺ cells in the CD45⁺mCherry^- fraction during leukemia development. g Percentage of NK1.1⁺ cells in the CD45⁺mCherry^- fraction during leukemia development. h Composition of B cell subpopulations during leukemia development (percentage in the B220⁺CD45⁺mCherry^- fraction). c-h Bone marrow cells were harvested from one femur and two tibias (Day 8, 10, 13, 15, and 16: n = 4 mice; Day 17: n = 3 mice; Day 20: n = 6 mice). i Representative images of immunostaining for collagen I (brown) from WT and mice at Day 8 and Day 20 post leukemia cell injection (scale bars, 50 μm) (left) and quantification of the percentage of collagen I per surface area (n = 3) (right). Throughout, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars represented mean ± SD