Fig. 2

Pre-B leukemia induces bone loss in vivo. a, b Bone marrow cells were harvested from one femur and two tibias after enzymatic dissociation (Day 8, 10, 13, 15, and 16: n = 4 mice; Day 17: n = 3 mice; Day 20: n = 6 mice). a Number of endothelial cells. b Number of osteoblastic cells. c Mean expression of osteocalcin (Bglap) in bone marrow CD45⁻ cells (WT), CD45⁺ (WT) and in CD45⁻ cells in leukemia-bearing mice at Day 16 post leukemia cell injection (n = 3). d 3D rendering of distal femur bone compartment (top panels: longitudinal sections; bottom panels: axial sections). Representative cortical and trabecular bones in the distal femurs of mice at stated days post leukemia cell injection (false colored images, n = 4 mice per time point). e–g Micro-CT analysis of femoral trabecular bone (n = 4 mice per time point). e Bone volume/total volume (BV/TV). f Trabecular spacing (Tb.Sp). g Trabecular number (Tb.N). h–i Micro-CT analysis of femoral cortical bone at trabecular zone (n = 4 mice per time point). h Cortical volume. i Cortical thickness. Throughout, *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Error bars represented mean ± SD