Fig. 3
From: Reduced expression but not deficiency of GFI1 causes a fatal myeloproliferative disease in mice
Gfi1 level rescue abrogates the myeloproliferative disease and myeloid leukemia predisposition. A Mouse model of Gfi1 level rescue. Mx-cre/KD mice injected with five doses of pIpC every 2 days have a deletion of the Neo cassette in the Human GFI1 transgene rescuing the level of Gfi1 (KI). B Representative FACS plot of BM cells from Mx-cre/KD mice injected with pIpC, WT, KD, and KO mice. BM cells were stained with Mac1 and Gr1 antibodies and analyzed by flow cytometry. C Survival curves of Mx-cre/KD mice injected or not with pIpC and KD mice injected with pIpC. Mice were considered sick when they showed symptoms such as weight loss, shaking, and less mobility. D Percentages of myeloid cells in sick KD mice injected with pIpC and Mx-cre/KD mice injected or not with pIpC. BM, blood, and splenic cells from sick mice were stained for the expression of myeloid markers Gr1 and Mac1 and analyzed by flow cytometry. E and F Proportions of T-ALL, myeloid leukemia, B-ALL, and undeterminate disease in sick Mx-cre/KD mice receiving or not pIpC and sick KD mice receiving also pIpC, 80 days after injections with ENU (E) or MMLV (F).
