Fig. 4

Sick KD mice showed increase myeloid progenitors in the BM and in the spleen. A and B Colony assay performed with 5000 BM cells (A) or 5000 splenic cells (B) from young KD (yKD) and KO (yKO), sick KD (sKD) and age-matched KO (oKO) mice. Every week, cells were harvested and plated in 1 mL of methylcellulose. C An equivalent number of CD45.1⁺ BM carrier cells and CD45.2⁺ BM cells from WT, KI, KD, and KO mice were transplanted into lethally irradiated recipient CD45.1 mice. After, 4, 8, 12, and 16 weeks, blood from transplanted recipient mice was analyzed for the expression of CD45.1 and CD45.2 by FACS. D A serial secondary transplantation assay was performed with BM cells from the 1st round transplanted mice. After, 4, 8, 12, and 16 weeks, blood from transplanted mice was analyzed for the expression of CD45.1 and CD45.2 by FACS.