Fig. 1

Acute and extended immune stimulation provokes conventional HSC markers. a Representative FACS plots, showing the staining of the Lineage⁻cKit⁺Sca1⁺ (LSK) compartment (left panels) and its dissection by CD150 and the Fgd5^mCherry reporter (mC, right panels) under control conditions (top) and under acute (middle) and extended (bottom) pIC stimulation, 24 h post stimulation. b Quantification of the frequencies of indicated cell populations: Lineage-cKit⁺Sca1⁻ (LK), Lineage^-cKit⁺Sca1⁺ (LSK), LSKCD150⁺mC⁻, and LSKCD150⁺mC⁺. Histograms indicate mean frequency and standard deviation from the bone-marrow mononuclear cells (% of total). c LSKCD150⁺mC⁺ are functional HSCs. Chimerism (% of gated CD45.2 cells) over time following acute or extended pIC-stimulated donors. Data are from at least five mice per histogram; *p < 0.05, **p < 0.01